Dear Marcia:

If you are trying to detect a rat IgM primary on mouse tissue, a very clean way
to go would be a Mouse x Rat IgM-biotin secondary antibody. Zymed just happens to
offer such a reagent (cat. no. 03-9840, clone: MARM-4). Please contact us if you
would like more details. Thanks.

Sincerely,

John Bliss

--

Marcia Bentz wrote:

> Thanks to all who responded to my question. I will call around and see if I
> can find an anti-rat IgM secondary that is mouse adsorbed. Let you know how
> it works out.
>
> 10:56 AM 10/19/2000 -0400, Connolly, Brett M wrote:
> >Marcia,
> >
> >If it were me, I would go with the rat anti-human CD77 followed by a
> >biotinylated anti-rat IgM that was preferably adsorbed against mouse tissue.
> >Many companies offer anti-rat IgM (ie. Jackson ImmunoResearch, Biogenex,KPL,
> >maybe Innogenex---Abizar are you there?). Call around and see if any are
> >mouse adsorbed. I use an anti-rat IgG from Biogenex that is mouse adsorbed,
> >maybe they have an IgM.
> >
> >I don't think that rat IgG will bind the rat IgM, it depends on how purely
> >it is prepared. However sometimes these secondaries are actually a mixture
> >of IgA, IgM, and IgG).
> >
> >I would also call tech services of the CD77 supplier and ask them if they
> >have any data on crossreactivity with the antibody.
> >
> >Lastly, when you decide to enroll in another immunology class, sign your
> >boss up too.
> >
> >Regards,
> >Brett
> >
> >
> >Brett M. Connolly, Ph.D.
> >Merck Research Laboratories
> >Department of Pharmacology
> >WP26A-3000
> >PO Box 4
> >West Point, PA 19486
> >Ph. 215-652-2501
> >FAX 215-652-2075
> >e-mail: brett_connolly@merck.com
> >
> >
> >> ----------
> >> From:        Marcia Bentz[SMTP:mb7x@virginia.edu]
> >> Sent:        Thursday, October 19, 2000 6:48 AM
> >> To:  histonet@pathology.swmed.edu
> >> Subject:     IHC with IgM
> >>
> >> Hello all,
> >> Ended up dropping my immunology class several years ago due to a serious
> >> illness and need the answer to a question. By boss is looking for an
> >> antibody CD77 (if wishes could come true, anti-mouse). Two problems arise,
> >> this is a IgM and I've only worked with IgG. I also only seem to be able
> >> to
> >> find mouse or rat anti-human CD77(I am staining mouse kidney). Since I use
> >> the Vector Elite kits will the rat IgG work with an IgM primary?. My boss
> >> insists it will, but well... he doesn't always know what he's talking
> >> about
> >> and has difficulty understanding IHC techniques sometimes.He also insists
> >> there will be no cross reactions with mouse or rat proteins. Can anyone
> >> answer this question for me?
> >>
> >> Thanks!
> >>
> >> Marcia
> >> Marcia Bentz
> >> Lab Specialist Sr
> >> University of Virginia
> >> Medicine/Nephrology
> >> mb7x@virginia.edu
> >> 804-982-1069
> >>
> >>
> >
> >
> >
> Marcia Bentz
> Lab Specialist Sr
> University of Virginia
> Medicine/Nephrology
> mb7x@virginia.edu
> 804-982-1069