RE: tissue shrinkage
|From:||Cynthia Favara <firstname.lastname@example.org>|
I think it would depend on a number of variables:
degree of hydration
processing times and reagents
fixation type and time
to name the ones that come to mind immediately. I would suggest a mini [Ha
Ha!!] experiment. Need enough specimens to be statically correct, measure
before an after crunch the numbers and there you have it.
Oh my goodness I have been in research far too long!!!! Good Luck
and I look forward to replies from others.
Rocky Mountain Laboratories
903 S. 4th Street
Hamilton, MT 59840
Sent: Wednesday, November 01, 2000 3:32 PM
Subject: tissue shrinkage
Once again we have an interesting question and I know someone out there has
a good answer.
Thanks in advance!!!
Any idea how much shrinkage from the original unfixed tissue size occurs
during paraffin embedding?
We are trying to do Morphometric measurements of a microvessel from inside
a human leg nerve.
We were under the impression that the dehydration step results in tissue
shrinkage of about 20%. Does
anybody have a "reference" to shrinkage?
Vanderbilt University Medical Center
Department of Plastic Surgery Research
Nashville, TN 37232-6904 USA
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