RE: tissue shrinkage

From:Cynthia Favara <>

	 I think it would depend on a number of variables:
			tissue type
			degree of hydration	
			processing times and reagents
			fixation type and time
to name the ones that come to mind immediately. I would suggest a mini [Ha
Ha!!] experiment. Need enough specimens to be statically correct, measure
before an after crunch the numbers and there you have it.
	Oh my goodness I have been in research far too long!!!! Good Luck
and I look forward to replies from others.

Cynthia Favara
Rocky Mountain Laboratories
903 S. 4th Street
Hamilton, MT 59840
FAX 406-363-9286

-----Original Message-----
Sent: Wednesday, November 01, 2000 3:32 PM
Subject: tissue shrinkage

Dear Histonetters,

Once again we have an interesting question and I know someone out there has
a good answer.

Thanks in advance!!!

Any idea how much shrinkage from the original unfixed tissue size occurs
during paraffin embedding?

We are trying to do Morphometric measurements of a microvessel from inside
a human leg nerve.

We were under the impression that the dehydration step results in tissue
shrinkage of about 20%.  Does
anybody have a "reference" to shrinkage?

Nancy Cardwell
Vanderbilt University Medical Center
Department of Plastic Surgery Research
Nashville, TN  37232-6904     USA
615-322-7266 phone
615-343-2050 fax

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