RE: stain prostate biopsy

From:"Viprino, Richard (JRP)" <>

We use carbol-Fuchsin from our AFB kit. Shows the biopsy nicely in the block
and the stain disappears with the routine H&E.
-----Original Message-----
From: HistoNet Server []
Sent: Sunday, October 22, 2000 1:01 AM
To: HistoNet Server
Subject: Daily Digest


Date: 21 Oct 2000 04:00:37 -0500
Subject: chemical formulas

Dear Histonetters,
does anybody know where one can found the structural formulas and chemical 
properties of the following dyes?
Alizarin Red
Trypan Blue
Neutral Red
Janus Green
Methylene Blue
Sudan Black
Nilus Blue

Thanks in advance to everybody


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Azienda Ospedaliera Istituti Ospitalieri di Verona


Date: 21 Oct 2000 08:45:12 -0500
From: Katri Tuomala <>
Subject: Re: chemical formulas

Hi Claudio,
Try following web page:



Date: 21 Oct 2000 12:00:32 -0500
From: "David Anderson" <>
Subject: Re: stain

We use an aqueous eosin made with potassium dichromate. I think the formula 
is in John Bancroft's book. The dichromate may not be any better or safer 
than the mercurochrome. Alcoholic eosin may work, but we haven't tried that.

David Anderson

>Subject: stain
>Date: Fri, 20 Oct 2000 22:17:50 -0400 (EDT)
>anyone know a good substitute for mercurochrome to stain prostate biopsies 

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Date: 21 Oct 2000 19:15:58 -0500
Subject: Re: prostate bxies

At our office, we use blue sponges and the Pathologist applies a small
of blue ink to the prostate biopsy.  This makes it easier to embed  and cut 

Jeanie Wade, H. T. (ASCP)  


Date: 21 Oct 2000 19:33:19 -0500
Subject: salary ranges

Can anyone tell me what the starting salary would be for a non-registered 
histotech with 5+ years experience.
Thanks in advance!


Date: 21 Oct 2000 21:15:58 -0500
From: denise M m Long-Woodward <>
Subject: Connecticut Society Meeting

The Connecticut Society of Histotechnologists will hold their annual Fall
meeting  Nov 4, 2000.  The meeting will be held at the West Haven VA 
Medical Center, in West Haven Connecticut from 8 AM - 1 PM.  There will
not be a vendor's exhibit at this event, however continental breakfast
and a lunch buffet are included.  Two speakers will present topics. One
topic is How to set up a recycling program by Terry Mattoon, HTL (ASCP).
Our second speaker is our " mystery speaker " but it is safe to say
he/she has some very valuable information to present for all histotechs.
If you can guess his/her name  you could win a UCONN Huskies T-shirt.   
I will be happy to fax or send the program announcement to anyone
interested in attending.
Denise Long Woodward, VP
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Date: 21 Oct 2000 21:45:11 -0500
Subject: marking prostate biopsy specimens

Several people have commented on marking prostate biopsy specimens for the 

Speaking as the end user, I don't care what you mark it with, as long as you

find all the pieces and tamp them flat in the paraffin!

I think that mercurochrome, though commonly used, is unacceptable because of

its high mercury content (26%). I've seen people get by with ordinary eosin.

Green Davidson marking ink looks horrible, but I've used it and it gets the 
job done, and is probably preferable when the embedder has to work without 
adequate light or magnification, or has impaired vision (all situations I
commonly in my travels).

The important thing is for the embedder to find all the pieces, each of
represents a moment of agony for the unfortunate patient. The person doing 
the grossing should be counting the cores and estimating their total length.

("three strips of white tissue with an aggregate length of about 60 mm")
not commonly done, but if I ran the zoo the person doing the grossing (or 
their assistant) would write these counts on a log sheet for the embedder.

Bob Richmond
Samurai Pathologist
Knoxville TN

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