RE: Oil Red O Question
From: | Joachim Siegmund <j.siegmund@pmail.net> |
Hi all,
because of the embedding procedure , the tissue has to go through <
alcohol, xylol etc >, there is no
fat left to detect. What you are basicly seeing, is, where the fat was!
The only thing, I can think of, is a fixation with osmium tetroxide.
The fat appears black under the microscope.
But remember, osmium tetroxide is extremly poison!!
Joachim Siegmund / BTA
>>>>>>>>>>>>>>>>>> Original Message <<<<<<<<<<<<<<<<<<
On 20.10.00, 12:19:56, Sarah Christo <schristo@cvm.tamu.edu> wrote
regarding RE: Oil Red O Question:
> Dear Betsy,
> The only way I know to do ORO on paraffin sections is to do the osmic
acid/potassium dichromate pre-treatment. It is in the AFIP manual.
> Sarah Christo, HT (ASCP)
> Research Associate, Histology Lab
> Texas A&M University
> College of Veterinary Medicine
> Dept. of Vet Anatomy & Public Health
> College Station, TX 77843-4458
> phone: (979) 845-3177
> fax: (979) 458-3499
> >>> "Molinari, Betsy" <BMolinari@heart.thi.tmc.edu> 10/19/00 10:19AM >>>
> Another ORO question. A pathologist wants ORO on paraffin sections. I
> have found one protocol for this method in the AFIP manual. Has anyone
> had any experience with this? It calls for the slides to be
> deparaffinized and hydrated to water,then placed in absolute propylene
> glycol for 3-5 mins. then stained in a 0.5%ORO solution for 48-72 hours.
> Thanks,
> Betsy Molinari
> Texas Heart Institute
> > ----------
> > From: J. A. Kiernan[SMTP:jkiernan@julian.uwo.ca]
> > Sent: Thursday, October 19, 2000 10:13 AM
> > To: Ryan.Linda
> > Cc: 'histonet'
> > Subject: Re: Oil Red O Question
> >
> > On Wed, 18 Oct 2000, Ryan.Linda wrote:
> >
> > > I need to get frozen sections from liver sections which have been
> > > stored in 10% NBF for some time now. Will I run into any problems
> > > freezing the livers at this stage? Any tips on Oil Red O with the
> > > pre-fixed frozen sections will be appreciated.
> >
> > It would be sensible to soak the block in 30% sucrose overnight
> > in the fridge (until they sink) for cryoprotection. This will
> > minimize damage from ice crystals, which can be the cause
> > of numerous holes in frozen sections. Otherwise just follow
> > the standard procedure for staining with oil red O.
> >
> > John A. Kiernan,
> > Department of Anatomy & Cell Biology,
> > The University of Western Ontario,
> > LONDON, Canada N6A 5C1
> >
> >
> >
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