RE: Oil Red O Question

From:"Molinari, Betsy" <BMolinari@heart.thi.tmc.edu>

Jeffrey,
I know, I thought the same thing when the pathologist requested it but
thought I would look anyway. It is on pg. 178 in the "AFIP Laboratory
Methods in Histology",1992.
Betsy Molinari
Texas Heart Institute

> ----------
> From: 	Jeffrey S Crews[SMTP:cruzetti@juno.com]
> Sent: 	Thursday, October 19, 2000 6:07 PM
> To: 	BMolinari@heart.thi.tmc.edu
> Cc: 	histonet@pathology.swmed.edu
> Subject: 	Re: Oil Red O Question
> 
> Are you sure that the protocol is for *paraffin* sections? I don't
> remember that from the AFIP manual, and the paraffin dehydration and
> clearing process should wash out all the fat present.
> 
> Jeffrey Crews, HTL (ASCP)
> 
> On Thu, 19 Oct 2000 12:19:52 -0500 "Molinari, Betsy"
> <BMolinari@heart.thi.tmc.edu> writes:
> >Another ORO question. A pathologist wants ORO on paraffin sections. I
> >have found one protocol for this method in the AFIP manual. Has 
> >anyone
> >had any experience with this? It calls for the slides to be
> >deparaffinized and hydrated to water,then placed in absolute 
> >propylene
> >glycol for 3-5 mins. then stained in a 0.5%ORO solution for 48-72 
> >hours.
> >Thanks,
> >Betsy Molinari
> >Texas Heart Institute
> >
> >> ----------
> >> From: 	J. A. Kiernan[SMTP:jkiernan@julian.uwo.ca]
> >> Sent: 	Thursday, October 19, 2000 10:13 AM
> >> To: 	Ryan.Linda
> >> Cc: 	'histonet'
> >> Subject: 	Re: Oil Red O Question
> >> 
> >> On Wed, 18 Oct 2000, Ryan.Linda wrote:
> >> 
> >> > I need to get frozen sections from liver sections which have been
> >> > stored in 10% NBF for some time now.  Will I run into any 
> >problems
> >> > freezing the livers at this stage?  Any tips on Oil Red O with 
> >the
> >> > pre-fixed frozen sections will be appreciated.
> >> 
> >>   It would be sensible to soak the block in 30% sucrose overnight
> >>   in the fridge (until they sink) for cryoprotection. This will
> >>   minimize damage from ice crystals, which can be the cause
> >>   of numerous holes in frozen sections. Otherwise just follow
> >>   the standard procedure for staining with oil red O.
> >> 
> >>  John A. Kiernan,
> >>  Department of Anatomy & Cell Biology,
> >>  The University of Western Ontario,
> >>  LONDON,  Canada  N6A 5C1
> >> 
> >> 
> >> 
> >
> 



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