RE: Oil Red O Question
|From:||"Molinari, Betsy" <BMolinari@heart.thi.tmc.edu>|
I know, I thought the same thing when the pathologist requested it but
thought I would look anyway. It is on pg. 178 in the "AFIP Laboratory
Methods in Histology",1992.
Texas Heart Institute
> From: Jeffrey S Crews[SMTP:firstname.lastname@example.org]
> Sent: Thursday, October 19, 2000 6:07 PM
> To: BMolinari@heart.thi.tmc.edu
> Cc: email@example.com
> Subject: Re: Oil Red O Question
> Are you sure that the protocol is for *paraffin* sections? I don't
> remember that from the AFIP manual, and the paraffin dehydration and
> clearing process should wash out all the fat present.
> Jeffrey Crews, HTL (ASCP)
> On Thu, 19 Oct 2000 12:19:52 -0500 "Molinari, Betsy"
> <BMolinari@heart.thi.tmc.edu> writes:
> >Another ORO question. A pathologist wants ORO on paraffin sections. I
> >have found one protocol for this method in the AFIP manual. Has
> >had any experience with this? It calls for the slides to be
> >deparaffinized and hydrated to water,then placed in absolute
> >glycol for 3-5 mins. then stained in a 0.5%ORO solution for 48-72
> >Betsy Molinari
> >Texas Heart Institute
> >> ----------
> >> From: J. A. Kiernan[SMTP:firstname.lastname@example.org]
> >> Sent: Thursday, October 19, 2000 10:13 AM
> >> To: Ryan.Linda
> >> Cc: 'histonet'
> >> Subject: Re: Oil Red O Question
> >> On Wed, 18 Oct 2000, Ryan.Linda wrote:
> >> > I need to get frozen sections from liver sections which have been
> >> > stored in 10% NBF for some time now. Will I run into any
> >> > freezing the livers at this stage? Any tips on Oil Red O with
> >> > pre-fixed frozen sections will be appreciated.
> >> It would be sensible to soak the block in 30% sucrose overnight
> >> in the fridge (until they sink) for cryoprotection. This will
> >> minimize damage from ice crystals, which can be the cause
> >> of numerous holes in frozen sections. Otherwise just follow
> >> the standard procedure for staining with oil red O.
> >> John A. Kiernan,
> >> Department of Anatomy & Cell Biology,
> >> The University of Western Ontario,
> >> LONDON, Canada N6A 5C1
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