Linda,
We wash our in water for about an hour before freezing the tissue.    Be
sure to use 
positive charged slides, so they won't wash off so easily.
Hazel

> -----Original Message-----
> From:	Ryan.Linda [SMTP:ryan2@niehs.nih.gov]
> Sent:	Wednesday, October 18, 2000 2:38 PM
> To:	'histonet'
> Subject:	Oil Red O Question
> 
> Hi!
> 
> I need to get frozen sections from liver sections which have been
> stored in 10% NBF for some time now.  Will I run into any problems
> freezing the livers at this stage?  Any tips on Oil Red O with the
> pre-fixed frozen sections will be appreciated.
> 
> Thanks,
> Linda
> 
> Linda Ryan, BS, HT(ASCP)HTL
> National Institute of Environmental Health Sciences
> 111  T. W. Alexander Dr.
> Bldg./rm.: 101/C-262
> P.O. Box 12233
> Research Triangle Park, NC 27709
> (919) 541-4880(Laboratory)
>