RE: Oil Red O Question
|From:||"Horn, Hazel" <HornHazelV@exchange.ach.uams.edu>|
We wash our in water for about an hour before freezing the tissue. Be
sure to use
positive charged slides, so they won't wash off so easily.
> -----Original Message-----
> From: Ryan.Linda [SMTP:email@example.com]
> Sent: Wednesday, October 18, 2000 2:38 PM
> To: 'histonet'
> Subject: Oil Red O Question
> I need to get frozen sections from liver sections which have been
> stored in 10% NBF for some time now. Will I run into any problems
> freezing the livers at this stage? Any tips on Oil Red O with the
> pre-fixed frozen sections will be appreciated.
> Linda Ryan, BS, HT(ASCP)HTL
> National Institute of Environmental Health Sciences
> 111 T. W. Alexander Dr.
> Bldg./rm.: 101/C-262
> P.O. Box 12233
> Research Triangle Park, NC 27709
> (919) 541-4880(Laboratory)
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