At the risk of saying me too.......

I have also only used either Citroclear or Histoclear EVER!!!! i have
never used xylene, and have had no problems at all.....

Emma Carter
Oxford BioMedica (UK) Ltd

> -----Original Message-----
> From:	Jan Shivers [SMTP:shive003@maroon.tc.umn.edu]
> Sent:	Wednesday, October 18, 2000 5:20 PM
> To:	HistoNet Server
> Subject:	Immunostaining and xylene substitutes
> 
> I have used nothing BUT xylene substitutes for quite a few years and
> have
> had excellent IHC staining results.  In the past I used Hemo De from
> Fisher,
> but that has recently been discontinued and replaced with Citrisolv
> (another
> limonene-based solvent).  I do four changes of xylene-like solvent, 3
> minutes each, before moving on to the graded alcohol series.
> 
> Jan Shivers
> Immunohistochemistry Lab
> Univ. of Minnesota Veterinary Diagnostic Medicine
>