RE: Immunostaining and xylene substitutes
|From:||Emma Carter <E.Carter@oxfordbiomedica.co.uk>|
At the risk of saying me too.......
I have also only used either Citroclear or Histoclear EVER!!!! i have
never used xylene, and have had no problems at all.....
Oxford BioMedica (UK) Ltd
> -----Original Message-----
> From: Jan Shivers [SMTP:email@example.com]
> Sent: Wednesday, October 18, 2000 5:20 PM
> To: HistoNet Server
> Subject: Immunostaining and xylene substitutes
> I have used nothing BUT xylene substitutes for quite a few years and
> had excellent IHC staining results. In the past I used Hemo De from
> but that has recently been discontinued and replaced with Citrisolv
> limonene-based solvent). I do four changes of xylene-like solvent, 3
> minutes each, before moving on to the graded alcohol series.
> Jan Shivers
> Immunohistochemistry Lab
> Univ. of Minnesota Veterinary Diagnostic Medicine
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