RE: Immunohistochemistry - Bone Morphogenic Proteins

I do staining with all the BMP's on formic acid decalcified bone and soft
tissue including tumor, paraffin processed, zinc formalin fixed.  I get all
my antibodies from Santa Cruz, they are Goat antibodies and I use them on
rat tissue.  No HIER, just a 5 min. digestion with proteanase k, they work
Patsy Ruegg

		-----Original Message-----
		From:	Lesley York []
		Sent:	Wednesday, November 01, 2000 5:05 AM
		Subject:	Immunohistochemistry - Bone Morphogenic

		   Is there anybody out there doing immunohistochemistry
(IHC) on 
		either frozen or formalin fixed tissue for Bone Morphogenic
		3,4 or 5? I am desperate for any information on every aspect
		where to get the antibodies, what methods work best,
fixation etc.
		My name is Lesley York and I work in the Anatomical Sciences

		dept. at the University of the Witwatersrand in
Johannesburg. Many 
		thanks - it's great to have this facility.

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