RE: Immuno Staining and Xylene Substitute
From: | "MacDonald, Jennifer" <jmacdonald@sach.org> |
We use only xylene substitutes on our processor and our staining setups. We
have had no problems with poor antigenicity because of this.
Jennifer MacDonald
> Substitute
>
> Hi everyone !
>
> Has anyone heard or read anything with regard to poor antigenicity when
> tissues are cleared using Xylene substitutes during processing or
> staining? Someone told me that they were told not to use xylene
> substitutes of any kind for tissue processing or for the deparaffinization
> and clearing steps in the staining process because it would effect immuno
> staining negatively. They did not have details as to how, what or why.
> Please enlighten me if you know anything with regard to this.
>
> Thank You,
> Mark Lewis
>
<< Previous Message | Next Message >>