RE: Immuno Staining and Xylene Substitute

From:"MacDonald, Jennifer" <>

We use only xylene substitutes on our processor and our staining setups.  We
have had no problems with poor antigenicity because of this.

Jennifer MacDonald

>  Substitute
> Hi everyone !
> Has anyone heard or read anything with regard to poor antigenicity when
> tissues are cleared using Xylene substitutes during processing or
> staining?  Someone told me that they were told not to use xylene
> substitutes of any kind for tissue processing or for the deparaffinization
> and clearing steps in the staining process because it would effect immuno
> staining negatively. They did not have details as to how, what or why.
> Please enlighten me if you know anything with regard to this.
> Thank You,
> Mark Lewis 

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