RE: Immuno Staining and Xylene Substitute
|From:||"MacDonald, Jennifer" <firstname.lastname@example.org>|
We use only xylene substitutes on our processor and our staining setups. We
have had no problems with poor antigenicity because of this.
> Hi everyone !
> Has anyone heard or read anything with regard to poor antigenicity when
> tissues are cleared using Xylene substitutes during processing or
> staining? Someone told me that they were told not to use xylene
> substitutes of any kind for tissue processing or for the deparaffinization
> and clearing steps in the staining process because it would effect immuno
> staining negatively. They did not have details as to how, what or why.
> Please enlighten me if you know anything with regard to this.
> Thank You,
> Mark Lewis
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