RE: Buffer Protection

From:Pam Marcum <>

Azide can also inhibit or retard some fluoresce dyes.
Pam Marcum

-----Original Message-----
From: Leek, Adrian []
Sent: Tuesday, October 17, 2000 12:52 PM
To: 'Luis Chiriboga'
Subject: RE: Buffer Protection

Azide is really only dangerous if you either drink it, or evaporate it to
dryness and then hit it very hard.  More important, it's not very effective
as a growth inhibitor.  Anaerobes are not affected, and some fungi can
actually use it as an energy source.
Adrian Leek,
CytoLogix Corp.

-----Original Message-----
From: Luis Chiriboga []
Sent: Monday, October 16, 2000 09:34 AM
To: Histonet
Subject: Buffer Protection

Hi all
I usually make large quantities of microwave solution on the order of
20L (I use a lot and I'm lazy). Has anyone ever added a preservative to
their buffer?  I occasionally get some growth (nothing bigger than an
occasional tadpole) in the carboy.  Azide is to dangerous, any other
recommendations? Has anyone ever had a problem with what they've added?
Thanks in advance

Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016

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