RE: Buffer Protection
|From:||Pam Marcum <email@example.com>|
Azide can also inhibit or retard some fluoresce dyes.
From: Leek, Adrian [mailto:ALeek@cytologix.com]
Sent: Tuesday, October 17, 2000 12:52 PM
To: 'Luis Chiriboga'
Subject: RE: Buffer Protection
Azide is really only dangerous if you either drink it, or evaporate it to
dryness and then hit it very hard. More important, it's not very effective
as a growth inhibitor. Anaerobes are not affected, and some fungi can
actually use it as an energy source.
From: Luis Chiriboga [mailto:firstname.lastname@example.org]
Sent: Monday, October 16, 2000 09:34 AM
Subject: Buffer Protection
I usually make large quantities of microwave solution on the order of
20L (I use a lot and I'm lazy). Has anyone ever added a preservative to
their buffer? I occasionally get some growth (nothing bigger than an
occasional tadpole) in the carboy. Azide is to dangerous, any other
recommendations? Has anyone ever had a problem with what they've added?
Thanks in advance
Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016
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