|From:||"Greer, Bonnie" <Bonnie.Greer@stjude.org>|
I did this along time ago.. but think standard 4 to 6 microns is Ok.
Bonnie G. Greer, HT (ASCP)
Histology Laboratory Manager
Animal Resources Center
Pager: 901-495-3578 (#2378)
From: Cheryl Crowder [mailto:firstname.lastname@example.org]
Sent: Wednesday, October 18, 2000 2:16 PM
Hi - I need some help. A researcher here wants to do autoradiography on
mouse tissue. He wants them paraffin embedded, cut and then the slides
given to him for the technique. I have never done any tissue for
Could someone tell me how thick the sections should be cut (they are
tagged with small amount of tritiium)? Are they put on regular, clean
slides or do you use charged or coated slides?
I don't have any information this and need it by tomorrow. Any help
be really appreciated.
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