I did this along time ago.. but think standard 4 to 6 microns is Ok.

Bonnie G. Greer, HT (ASCP)
Histology Laboratory Manager
Animal Resources Center
901-495-2272
FAX: 901-495-3112
Pager: 901-495-3578 (#2378)


-----Original Message-----
From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu]
Sent: Wednesday, October 18, 2000 2:16 PM
To: histonet@pathology.swmed.edu
Subject: Autoradiography


Hi - I need some help.  A researcher here wants to do autoradiography on 
mouse tissue.  He wants them paraffin embedded, cut and then the slides 
given to him for the technique.  I have never done any tissue for 
autoradio-graphy before.
	Could someone tell me how thick the sections should be cut (they are

tagged with small amount of tritiium)?  Are they put on regular, clean 
slides or do you use charged or coated slides?
	I don't have any information this and need it by tomorrow.  Any help
will 
be really appreciated.
Cheryl