RE: Alpha-feto Protein
From: | "Drew Sally A." <sa.drew@hosp.wisc.edu> |
We are using BioCare's polyclonal AFP...We use the pressure cooker with
BioCare's ReVeal buffer and put the slides on our Ventana ES/gen11...works
well with our tumor controls. What are you doing for a pretreatment with
the Dako Ab?
Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI
(608)265-6596
(608)263-1568(fax)
> -----Original Message-----
> From: Phyllis Davie [SMTP:pdavie@phenopath.com]
> Sent: Tuesday, October 24, 2000 7:52 PM
> To: Histonet
> Subject: Alpha-feto Protein
>
> My boss is unhappy with our current antibody to Alpha-1Feto Protein
> (AFP). We are currently using a rabbit polyclonal antibody from DAKO
> (cat#
> A0008). While it works admirably on fetal liver, he feels we are seeing
> only the tip of the iceberg on tumors such as hepatocellular carcinomas,
> hepatoblastomas, and some germ cell tumors.
> My question is: what antibodies are being used out there in
> Histonetland? What do you like about them and why? Any suggestions?
>
> Thanks for your help.
>
> Sincerely,
>
>
> Phyllis Davie
> Clinical Laboratory Supervisor
> PhenoPath Laboratories
> pdavie@phenopath.com
>
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