It is and ART!! RE: Sialidase Digestion

From:"Morken, Tim" <tim9@cdc.gov>

We've always said that Histotechnolgy is and art!

-----Original Message-----
From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com]
Sent: Tuesday, October 24, 2000 12:25 PM
To: HistoNet@pathology.swmed.edu
Subject: Re: Sialidase Digestion


Jeannette Mitchell at FAHC in Burlington, Vermont asks:

>>I have been asked to do an Alcian Blue with and without Sialidase 
Digestion.<<

To remove sialic acid (N-acetylneuraminic acid, NANA) from sections:

1. Deparaffinize sections and bring to water.

2. Sing NA NA Hey Hey Good-bye as loudly as you can for five minutes.

3. Proceed with Alcian blue stain.

Reference:
http://www.lyrichound.com/lyrics/e/everlast%20-%20goodbye.txt

     ;-)    ;-)    ;-)    ;-)    ;-)    ;-)    ;-)

SORRY ABOUT THAT FOLKS - I've been waiting to spring that one for about 25 
years!

Bob Richmond
Samurai Pathologist
Knoxville TN



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