|From:||Rebecca S Smith <firstname.lastname@example.org>|
We have a project that is continually giving us background problems. At
low dilutions of normal rabbit serum used as a control (for hyper-immune
rabbit serum) in lung tissue from sheep and human, we have background
staining of respiratory epithelial cells. We have tried blocking with: 5%
BSA, with 5% BSA with 20% normal goat serum(as that is the species of our
secondary), with 5% BSA with 2% casein, and Biogenix powerblock.
Does anyone have any good ways to inhibit background staining of normal
rabbit serum in sheep and/or human lung?
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