"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
Rena,<br>
<x-tab> </x-tab>Interesting
method. Any mention of the preferred fixative.<br>
Ian.<br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Thu, 19 Oct 2000 20:59:09
-0400<br>
From: Rena Fail <RFail@charleston.net><br>
Subject: Re: Tinctorial stains for mitochondria<br>
To: Darren Robinson <DRobinson@ciit.org><br>
Cc: histonet@pathology.swmed.edu<br>
<br>
Darren,<br>
<br>
You might want to try a Novelli's. It's
quick and it's easy. N<br>
hydrochloric acid (preheated to 60 degrees) for 3 minutes. Rinse in<br>
distilled water. 1% acid fuchsin for seconds, rinse in distilled water,
1%<br>
aqueous light green for app. 3 minuttes, rinse, dehydrate, clear, and
mount.<br>
Mitochondria stain purplish red and are surroundded by a green
colored<br>
peripheral wall. nuclear chromatin and collagen green, muscle
tissue<br>
purplish red,erythrocytes brilliant red.<br>
REF. Thompson Selected Histochemical and Histopathology Method.
1966,<br>
Charles C> Thomas, Publisher<br>
<br>
<br>
Rena Fail AS,HT(ASCP)<br>
Medical University of SC<br>
Charleston,Sc<br>
<br>
<br>
<br>
<br>
<br>
<br>
At 04:20 PM 10/19/00 -0400, you wrote:<br>
>I have some formalin fixed, formic acid decalcified tissues embedded
in wax<br>
>that I would like to section and stain for mitochondria. Does
anyone know<br>
>of any quick (non-immuno) staining methods that I could use. I
have read<br>
>that it is possible to detect them using an aniline acid-fuchsin
technique,<br>
>but I cannot find a supplier of this dye. Advice would be
appreciated.<br>
><br>
>Darren.<br>
><br>
><br>
><br>
></blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>