Fwd: Re: Tinctorial stains for mitochondria

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> Rena,<br> <x-tab>        </x-tab>Interesting method. Any mention of the preferred fixative.<br> Ian.<br> <br> <br> <blockquote type=cite class=cite cite>Date: Thu, 19 Oct 2000 20:59:09 -0400<br> From: Rena Fail <RFail@charleston.net><br> Subject: Re: Tinctorial stains for mitochondria<br> To: Darren Robinson <DRobinson@ciit.org><br> Cc: histonet@pathology.swmed.edu<br> <br>   Darren,<br> <br>       You might want to try a Novelli's. It's quick and it's easy.  N<br> hydrochloric acid (preheated to 60 degrees) for 3 minutes. Rinse in<br> distilled water. 1% acid fuchsin for seconds, rinse in distilled water, 1%<br> aqueous light green for app. 3 minuttes, rinse, dehydrate, clear, and mount.<br> Mitochondria stain purplish red and are surroundded by a green colored<br> peripheral wall. nuclear chromatin and collagen green, muscle tissue<br> purplish red,erythrocytes brilliant red.<br>  REF. Thompson Selected Histochemical and Histopathology Method. 1966,<br> Charles C> Thomas, Publisher<br> <br> <br>  Rena Fail AS,HT(ASCP)<br>  Medical University of SC<br>  Charleston,Sc<br> <br>  <br> <br> <br> <br> <br> At 04:20 PM 10/19/00 -0400, you wrote:<br> >I have some formalin fixed, formic acid decalcified tissues embedded in wax<br> >that I would like to section and stain for mitochondria.  Does anyone know<br> >of any quick (non-immuno) staining methods that I could use.  I have read<br> >that it is possible to detect them using an aniline acid-fuchsin technique,<br> >but I cannot find a supplier of this dye.  Advice would be appreciated.<br> ><br> >Darren.<br> ><br> ><br> ><br> ></blockquote> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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