"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
Cindy, <br>
<x-tab> </x-tab>Was just
about to reply when the posting from Kathy arrived. Full reference -
Lendrum. 1944 J.Path.Bact. <u>56</u>. 441.<br>
I agree with Kathy, it's a lovely stain on smears and tissues.<br>
Ian.<br>
<br>
<blockquote type=cite class=cite cite>Date: Wed, 25 Oct 2000 09:41:32
+1300<br>
From: Kathy Paton <Patonk@WHL.co.nz><br>
Subject: FW: chromotrope 2R for eosinophil granules<br>
To: "'histonet@pathology.swmed.edu'"
<histonet@pathology.swmed.edu><br>
<br>
<br>
<br>
-----Original Message-----<br>
From: Kathy Paton <br>
Sent: Wednesday, October 25, 2000 9:26 AM<br>
To: 'Cindy Burchell'<br>
Subject: RE: chromotrope 2R for eosinophil granules<br>
<br>
<br>
Dear Cindy<br>
Carbol-chromotrope method for eosinphils (Lendrum 1944)<br>
Reagent<br>
Phenol crystals (melt by gentle heat).......1g<br>
Chromotrope 2R ........................0.5g<br>
Mix<br>
Distilled water ........................100ml<br>
<br>
This stain keeps for about three months<br>
<br>
METHOD<br>
Hx as desired<br>
Place in staining solution for 30min<br>
Rinse in water<br>
DCM<br>
<br>
In my experience this is very selctive. Give it a go<br>
Kathy Paton<br>
Surgical Pathology Unit<br>
North Shore Hospital<br>
Auckland<br>
New Zealand<br>
<br>
-----Original Message-----<br>
From: Cindy Burchell
[<a href="mailto:burchell@facstaff.wisc.edu" eudora="autourl">mailto:burchell@facstaff.wisc.edu</a>]<br>
Sent: Wednesday, October 25, 2000 4:49 AM<br>
To: histonet@pathology.swmed.edu<br>
Subject: chromotrope 2R for eosinophil granules<br>
<br>
<br>
<x-tab> </x-tab>Hello
Histonetters,<br>
It's been a while since I have had the chance to be in
contact with <br>
you, and now I need a favor again. My bosses insist that I use
something <br>
called Chromotrope 2R to counterstain eosinophilic granules after IHC
<br>
staining MBP of eos. I've found references, but never any specific
<br>
methods. I would be in debt to anyone who could spell out just how
to <br>
handle this counter stain. Thanks so much in advance for your
time if you <br>
will help me in this matter!!! Cindy B.</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>