Fwd: FW: chromotrope 2R for eosinophil granules

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> Cindy, <br> <x-tab>        </x-tab>Was just about to reply when the posting from Kathy arrived. Full reference - Lendrum. 1944 J.Path.Bact. <u>56</u>. 441.<br> I agree with Kathy, it's a lovely stain on smears and tissues.<br> Ian.<br> <br> <blockquote type=cite class=cite cite>Date: Wed, 25 Oct 2000 09:41:32 +1300<br> From: Kathy Paton <Patonk@WHL.co.nz><br> Subject: FW: chromotrope 2R for eosinophil granules<br> To: "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu><br> <br> <br> <br> -----Original Message-----<br> From: Kathy Paton <br> Sent: Wednesday, October 25, 2000 9:26 AM<br> To: 'Cindy Burchell'<br> Subject: RE: chromotrope 2R for eosinophil granules<br> <br> <br> Dear Cindy<br> Carbol-chromotrope method for eosinphils (Lendrum 1944)<br> Reagent<br> Phenol crystals (melt by gentle heat).......1g<br> Chromotrope 2R     ........................0.5g<br> Mix<br> Distilled water    ........................100ml<br> <br> This stain keeps for about three months<br> <br> METHOD<br> Hx as desired<br> Place in staining solution for 30min<br> Rinse in water<br> DCM<br> <br> In my experience this is very selctive.   Give it a go<br> Kathy Paton<br> Surgical Pathology Unit<br> North Shore Hospital<br> Auckland<br> New Zealand<br> <br> -----Original Message-----<br> From: Cindy Burchell [<a href="mailto:burchell@facstaff.wisc.edu" eudora="autourl">mailto:burchell@facstaff.wisc.edu</a>]<br> Sent: Wednesday, October 25, 2000 4:49 AM<br> To: histonet@pathology.swmed.edu<br> Subject: chromotrope 2R for eosinophil granules<br> <br> <br> <x-tab>        </x-tab>Hello Histonetters,<br>     It's been a while since I have had the chance to be in contact with <br> you, and now I need a favor again.  My bosses insist that I use something <br> called Chromotrope 2R to counterstain eosinophilic granules after IHC <br> staining MBP of eos.  I've found references, but never any specific <br> methods.  I would be in debt to anyone who could spell out just how to <br> handle this counter stain.   Thanks so much in advance for your time if you <br> will help me in this matter!!!   Cindy B.</blockquote> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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