-----Original Message-----
From:	Frank Hogg [SMTP:FHogg@dchsystem.com]
<mailto:[SMTP:FHogg@dchsystem.com]> 
Sent:	Thursday, October 19, 2000 7:48 AM
To:	'histonet@pathology.swmed.edu'
Subject:	cu/zu

Is anyone using antibodies for copper/zinc or magnesium>SOD. For cu/zu I am
using 1:100, for mn I am using 1:20 both are polyclonal ab's. I am also
using heat retrieval. I am curious to know what others are doing.  PBS vs
Tris? I use sheep secondary, is there a better secondary for these? Any
suggestions would be appreciated.