for those setting up or doing double immunostaining: 

This review publication provided more answers to double immunostaining that
anything I have read to date and highly recommend it as a good read plus
practical protocol setup. 

van der Loos DM et al, Practical suggestions for successful immunoenzyme
double staining experiments, Histochem J 25:1-13, 1993

The author also has an excellent article in Oct 2000 J Histochem Cytochem
using DAKO ARK kit for double staining on human tissue samples.   Check out
the website  www.jhc.org.

Have a good weekend ---











Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303