nissl/nadph stain for glycerol-coverslipping

From:Susan Travers <>

We would like to counterstain napdh-reacted brain tissue with a nissl 
stain for cell bodies.  Because the nadph staining precipitates out a 
little with organic solvents, we find it best to use glycerol for 
coverslipping.  Thus, we need nissl stain that contrasts with the 
purple nadph, but won't be soluble in water. Any ideas out there?? 
thanks, susan travers
Susan Travers, Ph.D.
Associate Professor
Ohio State University
College of Dentistry
Columbus, Ohio  43210
Tel: (614)292-6366
Fax: (614)292-6087

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