microwave processing

From:"Steven E. Slap" <siksik@vgernet.net>

Hi Histonetters!

Wendy asked some basic questions about microwave processing of biopsies.
The process is generally done in three steps (dehydration, intermedium,
infiltration into paraffin), assuming that the biopsies are already
properly fixed.  In a 1000 watt microwave with appropriate temperature
control, the three-step process should take between 20 and 40 minutes.  I
prefer to use 100% ethyl alcohol for the dehydration step and isopropanol
for the intermedium.  The paraffin wax must be already melted (and, the
hotter the better), when placed in the microwave.

Best regards,
Steven Slap

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