I have several publications that do this, found in J Histochemistry and
Cytochemistry, go to www.jhc.com for a search. 

The new book by Shi and Taylor titled Antigen retrieval techniques:
applications in immunohistochemistry has a section on nonheating technics
for EM work.  Biotechniques book, ISBN 1-881299-43-0, or go their website
at www.biotechniques.com for particulars. This book has 400 pages and costs
$56.95, a deal at todays book prices.




Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303