Hi,
    DAKO p53 DO7 @ 1:400. Our control is a colon cancer that was formerly a case
that was stained positive with another control. We usually cut a few extra
slides on positive cases to use as controls.
    I believe the doctors sign them out on a 0,1+, 2+, 3+ basis.
Amos Brooks

"Van Eyck, Deb" wrote:

>
>     Hi all,
>         Are many labs running p53 routinely?? Whose antibody are you using??
> What control?
> As this is a research only antibody how is it being reported out-- Pos or
> neg??? Because of the gene over / under expression are these being
> graded???? Your input would be greatly appreciated.