Re: microwave processing

From:Debbie Pepperal <>


Hi, I have been using the Mega T/T microwave from Milestone. I have done a
series of small biopsies as well as the routine size sample for larger
specimens. The protocol works well, I am still in the process of optimising
the protocol.However, a small biopsy can be fixed , processed and embedded
and cut in 1 1/2 hours. The processing time can be reduced if the tissue is
well-fixed. I use neutral buffered formalin for fixation, JFC fluid for
dehydrating and then the usual paraffin wax for impregnation. this system
works on a "Time at Temperature" factor . feel free to contact me if you
need any more information,


HAPS, Newcastle, Australia.At 07:17 AM 30/09/2000 -0400, you wrote:
>Has anyone tried the microwave processing for biopsie tissue? If so what 
>procedures did you use. Length of time for final product to be put out. What 
>was the power of the micrwave? My Pathologistsmwould like to try this as
>as possible. So please can someone help.
>                                                         Thanks,
>                            Wendy                             

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