Re: blue mucin in H&E

From:Janice Mahoney <>

I've worked with Pathologists who have said that the H&E was not good unless the mucin stains pale blue.  Maybe you don't have a problem but have fixed one.

>>> a i d a n   s c h u r r <> 10/04/00 08:51PM >>>
Hi all,

We have recently switched to a linear staining system, which 
requires set, identical time in each solution.  To facilitate this, the 
concentration of the acid alcohol was lowered from 1% to 0.2%.  
We are now experiencing blue staining of mucin in cervical, colonic 
and gastric biopsies.  Any thoughts?!

Aidan, New Zealand
shin: device for finding furniture in the dark...
a i d a n   c   s c h u r r 
     section head,  histology department
      hutt valley health
       high street
        lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214

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