I have recently -- very recently -- learned about cutting vibratome sections
and I totally agree that 10-20 micron sections would be tough. I did 40
microns with great success on perfused brain, however.
Before I started I called Ted Pella and was advised to practice on
hard-boiled egg white. So off to the cafeteria I went to buy an egg and amid
lots of cracks about egg salad and the like I spent an afternoon practicing
and the next day I cut that brain like a pro! The consistency of hard-boiled
egg white was very similar to the perfused brain.
Practice. Yes, do practice.
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