Re: Subject: Freezing tissue, please fill in gaps
|From:||Gayle Callis <firstname.lastname@example.org>|
For some reason this message is partial, could Kim fill in gaps, please?
>Date: Tue, 03 Oct 2000 14:08:17 -0400
>From: Kim Kusser <email@example.com>
>Subject: Re: Subject: Freezing tissue
>X-MX-Comment: QUOTED-PRINTABLE message automatically decoded
>I've frozen tissues both ways, and, IMO, freezing with liquid nitorgen is
the same as with isopentane. I did identical tissues side by side using
both methods (mouse spleen, and lymph nodes), then did some stains
(CD3/B220, IgD/IgM), and found that the m
>Personally, I think Isopentane is a pain, and much prefer freezing on
liquid nitrogen. Also saves you the trouble of not keeping yet another
potentially dangerous chemical about. Not to mention the hazardous chemical
shipping charges for ordering the stu
>Just my opinion...
>Date: 2 Oct 2000 10:01:22 -0500
>From: "Vinnie Della Speranza" <firstname.lastname@example.org>
>Subject: Freezing tissue
>I would welcome your opinions and feedback.
>What is the optimal method of freezing tissues with liquid nitrogen? with or
>without isopentane and why?
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
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