<P> <BR>Dear Joan
<P>I did this back in the mid 90's with swine tissue. Frozen tissue, cleat from OCT, 1:500 dilution (we did do a titration run ranging from 1:100 to 1:5000) for 30 minutes, and an hour (the intensities were seen to be different), counterstain - if desired - mount with crystal mount and then permanent resin. Beautiful stain. Not so "beautiful" reagent to work with though.
<P>Good luck.
<P>Vikki Baker
<P>American Health Foundation
<P>Valhalla, New York
<P> <B><I>Joan Yonchek <Jyonchek@rtitechnology.com></I></B> wrote: <BR>
<BLOCKQUOTE style="BORDER-LEFT: #1010ff 2px solid; MARGIN-LEFT: 5px; PADDING-LEFT: 5px">Greetings,<BR><BR>I am interested in obtaining a protocol from anyone using the nuclear stain<BR>propidium iodide on formalin fixed frozen or paraffin embedded tissue.<BR><BR>Thanks,<BR>Joan<BR>RTI<BR></BLOCKQUOTE><p><br><hr size=1><b>Do You Yahoo!?</b><br>
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