Re: Fixatives for in-situ hybridization and RNA analysis
|From:||"L. Gibbs" <firstname.lastname@example.org>|
I have always used cross-linking fixatives for in-situ work,
preferably 4% paraformaldehyde. Many protocols call for a
proteinase K step. Just remember to keep everything RNAse-free!
Physiology & Biophysics
University of Washington
On Thu, 5 Oct 2000, Theleria Hackett wrote:
> Hello Histoneters!
> I'm looking for information on fixatives for in-situ hybridization and RNA
> analysis. Alcoholic formalin and Carnoys comes to my mind. They both have
> quick fixation time and minimal cross-linking. I have a principal
> investigator propose Para formaldehyde. Could I get some insight and help!
> Theleria R. Hackett
> Histology Supervisor
> Integrated Laboratory Systems
> (919) 544-4589 ext. 238
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