Hi Theleria-

I have always used cross-linking fixatives for in-situ work,
preferably 4% paraformaldehyde. Many protocols call for a
proteinase K step. Just remember to keep everything RNAse-free!

Good Luck!

Lorraine Gibbs
Physiology & Biophysics
University of Washington

On Thu, 5 Oct 2000, Theleria Hackett wrote:

> Hello Histoneters!
> I'm looking for information on fixatives for in-situ hybridization and RNA
> analysis.  Alcoholic formalin and Carnoys comes to my mind.  They both have
> quick fixation time and minimal cross-linking.  I have a principal
> investigator propose Para formaldehyde.  Could I get some insight and help!
> 
> Thanks,
> 
> Theleria R. Hackett
> Histology Supervisor
> Integrated Laboratory Systems
> (919) 544-4589 ext. 238
> thackett@ils-inc.com
> 
> 
> 
>