Re: Buffer Protection

From:Amy Porter <>

Luis -Our lab uses a very reliable 25X buffer from
ScyTek products for life science.  Not sure what type
of buffer you are looking for - they have a large
variety of buffer products.  We use their tbs/tbs+tw20
for immuno.  Already has preservatives added.  No muss
no fuss.  They offer concentrates in varying degrees
to make up to 250L. The pH stays stable & we dilute
with sterile water, no growth problems.  Contact me if
you would like more info.
--- Luis Chiriboga <> wrote:
> Hi all
> I usually make large quantities of microwave
> solution on the order of
> 20L (I use a lot and I'm lazy). Has anyone ever
> added a preservative to
> their buffer?  I occasionally get some growth
> (nothing bigger than an
> occasional tadpole) in the carboy.  Azide is to
> dangerous, any other
> recommendations? Has anyone ever had a problem with
> what they've added?
> Thanks in advance
> --
> Luis Chiriboga Ph.D HT(ASCP)QIHC
> Department of Pathology 4W27
> Bellevue Hospital
> NYU School of Medicine
> First Avenue & 27th Street
> New York, NY 10016
> (212)562-4667

Amy S.Porter, HT(ASCP)
Michigan State University
College of Human Medicine

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