RE: staining plastic-embedded sections
From: | "Bennett, Catherine (Katie)" <cbennett@lrri.org> |
Roger Moretz wrote:
I can't speak to all of the stains mentioned, but I can say that H&E,
Masson's, PAS and methyl green-pyronin all work on JB4/gma sections at 1 to
5 microns. There are some caveats. First, the staining is much lighter for
thinner sections (go fig :-)).......
One reason the staining is much lighter in GMA than in paraffin is that the
sections are thinner, therefore there is less tissue to pick up the stains.
*********************************
Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico
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