RE: staining plastic-embedded sections

From:"Bennett, Catherine (Katie)" <>

Roger Moretz wrote:

I can't speak to all of the stains mentioned, but I can say that H&E,
Masson's, PAS and methyl green-pyronin all work on JB4/gma sections at 1 to
5 microns.  There are some caveats.  First, the staining is much lighter for
thinner sections (go fig :-)).......

One reason the staining is much lighter in GMA than in paraffin is that the
sections are thinner, therefore there is less tissue to pick up the stains.

Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico

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