|From:||"Philopena, Jennifer" <firstname.lastname@example.org>|
I've used Novocastra's CM1 anti-p53 to detect wt transgene expression in
transduced rodent tissue. Very clean antibody (for this purpose).
Microwave HIER, 60min incubation with primary at 1:1000.
I also used Calbiochem's Ab-6 to detect wt and mutated p53 expression in
I hope this helps.
Jennifer M. Philopena
3525 John Hopkins Court
San Diego, CA 92121
> From: Van Eyck, Deb
> Sent: Tuesday, October 10, 2000 2:01 PM
> To: 'email@example.com.'
> Subject: p53
> Hi all,
> Are many labs running p53 routinely?? Whose antibody are you
> What control?
> As this is a research only antibody how is it being reported out-- Pos or
> neg??? Because of the gene over / under expression are these being
> graded???? Your input would be greatly appreciated.
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