Hi all.
I've used Novocastra's CM1 anti-p53 to detect wt transgene expression in
transduced rodent tissue.  Very clean antibody (for this purpose).
Microwave HIER, 60min incubation with primary at 1:1000.
I also used Calbiochem's Ab-6 to detect wt and mutated p53 expression in
vitro.
I hope this helps.  

Jennifer M. Philopena
Scientist 1
Canji, Inc.
3525 John Hopkins Court
San Diego, CA 92121
jennifer.philopena@canji.com

> ----------
> From: 	Van Eyck, Deb
> Sent: 	Tuesday, October 10, 2000 2:01 PM
> To: 	'histonet@pathology.swmed.edu.'
> Subject: 	p53
> 
> 	
>     Hi all,
>         Are many labs running p53 routinely?? Whose antibody are you
> using??
> What control?
> As this is a research only antibody how is it being reported out-- Pos or
> neg??? Because of the gene over / under expression are these being
> graded???? Your input would be greatly appreciated.
>