RE: melanin bleach query

From:Emma Carter <>

> One of our pathologists needs a melanin bleach performed on sections
> of
> lymph node requiring IHC (S-100 and HMB45). From previous knowledge, I
> recollect that if done prior to the IHC stain, the tissue washes off,
> however, by doing it post IHC, would the chromagen (DAB) still be
> intact?
> Will there be any fading of positive staining due to this procedure.
	Have you considered using a nickel or cobalt step to turn the
DAB black instead of staying brown....

	emma carter
	Oxford BioMedica (UK) Ltd

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