We have much better success with a progressive stain, although our stainer
is not linear.  Much more consistent, and you can avoid the acid alcohol.

Jennifer

> ----------
> From: 	a i d a n   s c h u r r[SMTP:Aidan.Schurr@hvh.co.nz]
> Sent: 	Wednesday, October 04, 2000 8:51 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	blue mucin in H&E
> 
> Hi all,
> 
> We have recently switched to a linear staining system, which 
> requires set, identical time in each solution.  To facilitate this, the 
> concentration of the acid alcohol was lowered from 1% to 0.2%.  
> We are now experiencing blue staining of mucin in cervical, colonic 
> and gastric biopsies.  Any thoughts?!
> 
> Cheers
> Aidan, New Zealand
> ___________________________________________________
> shin: device for finding furniture in the dark...
> ___________________________________________________
> a i d a n   c   s c h u r r 
>      section head,  histology department
>       hutt valley health
>        high street
>         lower hutt, new zealand
> 
>      ph.  ++64 4 5709173
>      fax  ++64 4 5709214
> ___________________________________________________
> 
>