RE: blue mucin in H&E
|From:||Bert Dotson <email@example.com>|
We have adjusted several protocols over the years for this type of
automation. Most recently we dispensed with a regressive technique and were
able to improve consistency and shorten the staining protocol by about 15
Two suggestions: If you wish to stay with a regressive technique, try
substituting aqueous acetic acid for acid alcohol. You may have to play
with the concentration a bit but consistency is generally improved because
small changes in the acetic concentration do not have the same impact as
small changes in acid alcohol concentration--basically its more forgiving.
When you are working with very weak acid alcohol it is difficult to
maintain the same exact concentration from day to day.
Second, the mucin staining is a function of the Hematoxylin pH. Establish a
rigorous QC for Hematoxylin before use. Lowering the pH (usually by adding
acetic acid) can significantly reduce mucin staining. Automation of this
type can really exaggerate even small differences in the Hematoxylin.
noticeable differences occur with pH changes of as small as 0.2 either
From: a i d a n s c h u r r [SMTP:Aidan.Schurr@hvh.co.nz]
Sent: Wednesday, October 04, 2000 9:52 PM
Subject: blue mucin in H&E
We have recently switched to a linear staining system, which
requires set, identical time in each solution. To facilitate this, the
concentration of the acid alcohol was lowered from 1% to 0.2%.
We are now experiencing blue staining of mucin in cervical, colonic
and gastric biopsies. Any thoughts?!
Aidan, New Zealand
shin: device for finding furniture in the dark...
a i d a n c s c h u r r
section head, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
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