|From:||"Barry, Lilith" <Lilith.Barry@nrc.ca>|
From my experience it would be next to impossible to cut so thin consistent
sections with vibrotome.
What I would recommend is to cryoprotect your tissue, embed it in O.C.T.
compound and cut it with cryostat.
I also think that 10-20 micron sections would be little too delicate for
free floating work.
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National Research Council
Institute of Biological Sciences
From: Joan Yonchek [mailto:Jyonchek@rtitechnology.com]
Sent: Friday, September 29, 2000 5:17 PM
I am interested in hearing from anyone versed in the use of a Vibratome.
I have been asked to obtain 10-20 micron thick sections of small pieces (1mm
cubes) of formalin fixed skin. Specifically I would like to know the speed,
amplitude and blade angle you would recommend as well as the method for
embedding the tissue in agarose. Also, do you use a sapphire knife, a
single edge injector type razor blade or a double edge type razor blade? Is
it necessary to remove the agarose from the section before staining? What
slides are best? Do you prefer staining the free-floating section and then
adhering it to the slide or dry the section on the slide before staining?
Other than that I got it covered!
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