RE: Helicobacter Pylori Stains

From:"Tom T. McNemar" <>

We recently switched from the Warthin Starry stain to immuno.  The Warthin
Starry was a quick stain but it gave inconsistent results and had to be
watched pretty closely.  I much prefer (as do the Pathologists) the immuno.
Yes, it takes longer and costs a little more on the surface but when you
figure in the time and reagents involved in the frequent repeats I think it
balances out pretty well.  We used to batch the Warthin Starry and do it
only twice a week.  With immuno we are able to do them daily so the
reporting is better.  The immuno is consistently very good with intense
staining.  We are using Dako concentrated ab with Dako target retrieval in
the steamer for 30 minutes.

Tom Mc Nemar, HT(ASCP)
Pathology Supervisor
Licking Memorial Hospital
Newark, Ohio

> -----Original Message-----
> From:	Richard Maliniak []
> Sent:	Monday, October 02, 2000 5:33 PM
> To:	HistoNet Server
> Subject:	Helicobacter Pylori Stains
> We currently use the 'Genta' stain for H. Pylori.  We are not satisified
> with the stain and are thinking of doing 'ALL' H.Pylori requested with
> immunohistochemistry.  The questions I have been asked to answer are the
> following:
> 1. What is the quickest and most reliable H.Pylori stain...immuno or
> special stain? 2. What is the fastest anyone can do an immunostain for
> H.Pylori?  
> 3. Are tech and reagent costs similar for  'rapid' H.Pylori via
> immunoperoxidase and special stains?
> Thanks in advance,
> Rick << File: Card for Maliniak, Richard >> 

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