RE: Freezing tissue
|From:||"Macke, Gail" <email@example.com>|
I disagree with you on how long it takes to freeze with liquid nitrogen.
Please don't put your hands in or on liquid nitrogen!!!!!! It took less
than a second for me to get second degree burns on my left thumb and index
finger in December of 1997. It was a very painful event and one I
personally never let happen to me again. Liquid nitrogen will freeze warm
objects faster than blinking your eye.
Gail Macke, HTL
Shriners Hospital for Children: Shriners BURNS Hospital--Cincinnati,
P.S. Yes, I did get a lecture on chemical burns, believe I did.
> -----Original Message-----
> From: Jim at ProSciTech [SMTP:firstname.lastname@example.org]
> Sent: Wednesday, October 04, 2000 7:16 AM
> To: 'Greer, Bonnie'; 'Vinnie Della Speranza';
> Subject: RE: Freezing tissue
> Sorry Vinnie, better ask for a refund on your tuition- or was that a slip
> the pen? The gas forming around a warm object inserted into liq N2 forms
> insulating envelope (Leidenfrost). Freezing speed in liq N2 is slower than
> near freezing point Iso-pentane.
> You can stick a finger into liq N2 for a couple of seconds and have no
> but don't try that in cold iso-pentane.
> Faster freezing results in smaller (ultimately no) ice crystals. It's
> crystals that destroy tissues.
> For the light microscopist the goal is to have those crystals small enough
> not cause tissue damage that is visible under the light microscope. There
> numerous methods to attain that goal.
> Jim Darley
> ProSciTech Microscopy PLUS
> PO Box 111, Thuringowa QLD 4817 Australia
> Ph +61 7 4774 0370 Fax:+61 7 4789 2313 email@example.com
> Great microscopy catalogue, 500 Links, MSDS, User Notes
> ABN: 99 724 136 560 www.proscitech.com
> On Wednesday, October 04, 2000 7:59 AM, Greer, Bonnie
> [SMTP:Bonnie.Greer@stjude.org] wrote:
> > I did muscle bx`s for a long time I used a dewar filled with liquid
> > and I used another container filled with the isopentane.... suspended
> > until frozen. You thaw just enough to freeze your tissue for 20
> seconds. I
> > was taught that the isopentane insulates the tissue causing lee artifact
> > .Email me if I can help.
> > -----Original Message-----
> > From: Vinnie Della Speranza [mailto:firstname.lastname@example.org]
> > Sent: Monday, October 02, 2000 9:51 AM
> > To: email@example.com
> > Subject: Freezing tissue
> > I would welcome your opinions and feedback.
> > What is the optimal method of freezing tissues with liquid nitrogen?
> with or
> > without isopentane and why?
> > thanks,
> > Vinnie
> > Vinnie Della Speranza
> > Manager for Anatomic Pathology Services
> > Medical University of South Carolina
> > 165 Ashley Avenue
> > Suite 309
> > Charleston, SC 29425
> > ph: (843) 792-6353
> > fax: (843) 792-8974
> > email: Dellav@musc.edu
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