RE: Freezing tissue
|From:||Jim at ProSciTech <firstname.lastname@example.org>|
Sorry Vinnie, better ask for a refund on your tuition- or was that a slip of
the pen? The gas forming around a warm object inserted into liq N2 forms an
insulating envelope (Leidenfrost). Freezing speed in liq N2 is slower than in
near freezing point Iso-pentane.
You can stick a finger into liq N2 for a couple of seconds and have no harm,
but don't try that in cold iso-pentane.
Faster freezing results in smaller (ultimately no) ice crystals. It's those
crystals that destroy tissues.
For the light microscopist the goal is to have those crystals small enough to
not cause tissue damage that is visible under the light microscope. There are
numerous methods to attain that goal.
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On Wednesday, October 04, 2000 7:59 AM, Greer, Bonnie
> I did muscle bx`s for a long time I used a dewar filled with liquid nitrogen
> and I used another container filled with the isopentane.... suspended inside
> until frozen. You thaw just enough to freeze your tissue for 20 seconds. I
> was taught that the isopentane insulates the tissue causing lee artifact
> .Email me if I can help.
> -----Original Message-----
> From: Vinnie Della Speranza [mailto:firstname.lastname@example.org]
> Sent: Monday, October 02, 2000 9:51 AM
> To: email@example.com
> Subject: Freezing tissue
> I would welcome your opinions and feedback.
> What is the optimal method of freezing tissues with liquid nitrogen? with or
> without isopentane and why?
> Vinnie Della Speranza
> Manager for Anatomic Pathology Services
> Medical University of South Carolina
> 165 Ashley Avenue
> Suite 309
> Charleston, SC 29425
> ph: (843) 792-6353
> fax: (843) 792-8974
> email: Dellav@musc.edu
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