Kenneth,

	In our publication "Alterations in transforming growth factor-alpha
and epidermal growth factor receptor expression during rat esophageal
tumorigenesis" (Mol Carcinogenesis 15:144-153, 1996), we used a monoclonal
mouse anti-human EGFR antibody from Sigma diluted 1:100 on formalin-fixed
paraffin embedded rat esophagus.

Carol
_________
Carol L. Sabourin, Ph.D.
Battelle
Medical Research and Evaluation Facility
505 King Avenue
Columbus, OH 43201-2693
Phone: (614) 424-5024
sabourinc@battelle.org
>  
	 
	Kenneth Wester wrote:
>  
> Date: 10 Oct 2000 02:15:24 -0500
> From: Kenneth Wester <kenneth.wester@genpat.uu.se>
> Subject: Antibody to rat-EGFR?
> 
> Hi Histonet members.
> 
> Is there anyone who have experience from immunohistochemical stainings of
> epidermal growth factor receptor (EGFR) in formalin-fixed,
> paraffin-embedded rat tissue? In my initial tests, I have used a rabbit
> polyclonal anti-EGFR from Santa Cruz Biotechnology (1005, sc03). Some
> confusing staining results make me want to confirm these with a second
> antibody. However, there seem to be difficult to find commercially
> available rat-EGFR antibodies. Greatful for any tips and advice in how to
> proceed.
> 
> 
> Kenneth Wester, Ph.D.                  	phone:  +46 (0)18 611 02 06
> 
> Dept. of Genetics & Pathology		fax:    +46 (0)18 471 3432
> The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjoldsvag 20
> S-752 37 Uppsala, Sweden
> 
> Kenneth Wester, Ph.D.                  	phone:  +46 (0)18 611 02 06
> 
> Dept. of Genetics & Pathology		fax:    +46 (0)18 471 3432
> The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjoldsvag 20
> S-752 37 Uppsala, Sweden
> 
> 
> 
>