RE: B5 fixative and immunos
From: | Patsy.Ruegg@UCHSC.edu |
I agree with this statement. Since I switched by fixative from 10%NBF to
Zinc Formalin (similar to B5, but Zinc instead of Mercury), I have almost
illimanated the need for any HEIR, I use only mild enzyme digestion on
occasion.
Patsy Ruegg
-----Original Message-----
From: DDittus787@aol.com [mailto:DDittus787@aol.com]
Sent: Thursday, October 12, 2000 4:13 PM
To: dsitrin@unipathllc.com; histonet@pathology.swmed.edu
Subject: Re: B5 fixative and immunos
Dear Donna
Usually B5 tissue does not need retrieval, it usually
stains with the CD
markers real well, it is my experience that when we
retrieved B5 fixed tissue
we lost most to all of the antigenicity.
Dana
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