Non-specific immuno staining

From:"Nocito, Joseph" <>

Hey all,
don't you just love Friday 13th that has a full moon?  I begging for help.
I ran a set of immunos on a fine-needle aspirate from the adrenal area
(could be adrenal, could be kidney, could be liver). It was fixed in
formalin.  The first run of immnos, I noticed the negative control was
reacting.  I called the pathologost and he told me it could be any of the
above mentioned organs.  I recut the block and performed an avidin-biotin
block.  Guess what? Yep, negative control was staining again.  The
avidin-biotin blocking kit is new. Any thoughts on this?  I don't, my two
synapses are exhausted, it's tough keeping them going.  Thanks for your help
and have a good, safe weekend

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

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