How much of the tissue would you want on your section?

From:Anila Syed <syedab@totalise.co.uk>

Hello Histonetters,

I'm just starting to section mouse livers for the first time (all my experience being with brain tissue and white matter so far).

My question is, how much of the liver would you want to get on your section? Is it necessary to try and get the whole thing.

The tissue is wax embedded and I am doing IHC

Many thanks for any help

:)

Anila Syed




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