|From:||Bert Dotson <firstname.lastname@example.org>|
Just a couple of follow-up comments on my earlier post.
1) In our lab with our formulation of hematoxylin, we have obtained
exceptional results with hematoxylin pH in the range of 1.8-2.0. This is
lower than the acceptable range commonly reported.
2) During a period of 5 years we used a regressive technique with 0.1 % HCL
in 70% EtOH for differentiation and ammonia water for bluing. Almost all of
the slides from this period exhibit seriously faded nuclear staining. A
little more than three years ago we went to a more controllable progressive
stain with a modified Harris Hematoxylin at pH 2.0 and blue in running
water only. These slides have not faded. Granted this is a completely
empirical observation with no scientific control and I have no potential
explanation for why this might have occurred.
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