HEMATOXYLIN ORDER

From:Lee & Peggy Wenk <lpwenk@mail.netquest.com>

Does it matter what order the reagents are added together to
make an aluminum salt-sodium iodate hematoxylin, such as
Mayer?

I always add them in the same order, with lots of stirring
between reagents. Got wondering if it would make a difference 
if they were added in a different order. Since we're making
hematoxylin into hematein, and making hematein into
aluminum-hematein, it seems like it should make a difference.

(This is for my School. I still have the students make Mayer
hematoxylin. It helps them to learn what's in their
hematoxylin, and hopefully helps them learn to 
troubleshoot.

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073




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