FW: Vimentin Immunostaining

From:"Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>



> -----Original Message-----
> From:	Hewlett Bryan [SMTP:HEWLETT@HHSC.CA]
> Sent:	Thursday, October 05, 2000 9:26 AM
> To:	'Nocito, Joseph'
> Subject:	RE: Vimentin Immunostaining
> 
> Joe, 
> 
> I'm sure that's not true! 
> My original reply to the group was not posted for some reason, so I will
> share it with you. If you could forward it to histonet, I would be
> grateful.
> 
> The use of vimentin staining as an indicator of antigen availability was,
> IMHO, always a naive premise. The fact that one epitope on an antigen is
> available for immunostaining does NOT mean that another epitope on the
> same or any other antigen is!!!  Vimentin was chosen because of the
> masking induced by prolonged...... ( what a joke!!  24-72 hour is
> prolonged? read J.R.Baker, Principles of biological microtechnique, the
> bible!)....... NBF fixation ( wrongly called overfixation, actually
> correct fixation!). 
> 
> These were the days PRIOR to HIER. The idea persists today, even the NCCLS
> Quality Assurance for Immunocytochemistry guideline continues to
> perpetuate this myth.
> 
> As you so correctly point out, HIER is necessary for both the V9 and 3B4
> clones to work appropriately. In fact, we have done fixation time point
> experiments on NBF fixed tissues from 2 hours fixation to 4 months
> fixation. Not a single observer, who has seen the vimentin stains on these
> tissues, can detect ANY difference in immunoreactivity. I wish I could say
> that about some other antibodies applied to these tissue!
> 
> Save your time, reagents and money, restrict the vimentin staining to
> cases on which it may really be useful. 
> 
> OK! 
> Rant over, flame away guys. 
> 
> Bryan 
> 
> 
> 	---------- 
> From:   Nocito, Joseph[SMTP:joseph_nocito@srhc.iwhs.org] 
> Sent:   October 5, 2000 8:33 AM 
> To:     Hewlett Bryan 
> Subject:        RE: Vimentin Immunostaining 
> 
> 	did I say peace of mind?  I meant to say a piece of my mind.  Not
> that I 
> have much left to give 
> 
> 	Joe 
> 
> 	> -----Original Message----- 
> > From: Hewlett Bryan [SMTP:HEWLETT@HHSC.CA] 
> > Sent: Thursday, October 05, 2000 7:37 AM 
> > To:   'terij@prlnet.com'; histonet; 'Nocito, Joseph' 
> > Subject:      RE: Vimentin Immunostaining 
> > 
> > Joe, 
> > 
> > Is that the same peace of mind that Admiral Nelson felt? 
> > As he urged his ships into battle, his aids complained of overwhelming 
> > numbers of enemy ships, Nelson immediately placed the telescope to his 
> > blind eye and exclaimed; Ships? I see no ships! 
> > 
> > Bryan 
> > 
> >       ---------- 
> > From:   Nocito, Joseph[SMTP:joseph_nocito@srhc.iwhs.org] 
> > Sent:   September 28, 2000 2:22 PM 
> > To:     'terij@prlnet.com'; histonet 
> > Subject:        RE: Vimentin Immunostaining 
> > 
> >       Teri, 
> > we perform a vimentin on those cases that were processed at another 
> > facility 
> > also.  We had stopped this for a while, but we were getting erroneous 
> > results, so we started adding a vimentin again just to give us peace of 
> > mind. 
> > 
> >       Joe Nocito, B.S., HT(ASCP)QIHC 
> > Histology Supervisor 
> > Christus Santa Rosa Hospitals 
> > San Antonio, Texas 
> > 
> >       > -----Original Message----- 
> > > From: terij@prlnet.com [SMTP:terij@prlnet.com] 
> > > Sent: Thursday, September 28, 2000 11:31 AM 
> > > To:   histonet 
> > > Subject:      Vimentin Immunostaining 
> > > 
> > > We currently are doing a Vimentin immunostain on every IHC case we
> have 
> > to 
> > > access antigenicity.  Is anyone else out there doing this?  Is it
> really 
> > 
> > > necessary or helpful, especially considering one needs to antigen 
> > retrieve 
> > > this in order for it to work? 
> > > 
> > > Thanks, 
> > > 
> > > Teri Johnson 
> > > Physician's Reference Laboratory 
> > > Overland Park, KS 
> > > 
> > 
> 



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