FW: Vimentin Immunostaining
From: | "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> |
> -----Original Message-----
> From: Hewlett Bryan [SMTP:HEWLETT@HHSC.CA]
> Sent: Thursday, October 05, 2000 9:26 AM
> To: 'Nocito, Joseph'
> Subject: RE: Vimentin Immunostaining
>
> Joe,
>
> I'm sure that's not true!
> My original reply to the group was not posted for some reason, so I will
> share it with you. If you could forward it to histonet, I would be
> grateful.
>
> The use of vimentin staining as an indicator of antigen availability was,
> IMHO, always a naive premise. The fact that one epitope on an antigen is
> available for immunostaining does NOT mean that another epitope on the
> same or any other antigen is!!! Vimentin was chosen because of the
> masking induced by prolonged...... ( what a joke!! 24-72 hour is
> prolonged? read J.R.Baker, Principles of biological microtechnique, the
> bible!)....... NBF fixation ( wrongly called overfixation, actually
> correct fixation!).
>
> These were the days PRIOR to HIER. The idea persists today, even the NCCLS
> Quality Assurance for Immunocytochemistry guideline continues to
> perpetuate this myth.
>
> As you so correctly point out, HIER is necessary for both the V9 and 3B4
> clones to work appropriately. In fact, we have done fixation time point
> experiments on NBF fixed tissues from 2 hours fixation to 4 months
> fixation. Not a single observer, who has seen the vimentin stains on these
> tissues, can detect ANY difference in immunoreactivity. I wish I could say
> that about some other antibodies applied to these tissue!
>
> Save your time, reagents and money, restrict the vimentin staining to
> cases on which it may really be useful.
>
> OK!
> Rant over, flame away guys.
>
> Bryan
>
>
> ----------
> From: Nocito, Joseph[SMTP:joseph_nocito@srhc.iwhs.org]
> Sent: October 5, 2000 8:33 AM
> To: Hewlett Bryan
> Subject: RE: Vimentin Immunostaining
>
> did I say peace of mind? I meant to say a piece of my mind. Not
> that I
> have much left to give
>
> Joe
>
> > -----Original Message-----
> > From: Hewlett Bryan [SMTP:HEWLETT@HHSC.CA]
> > Sent: Thursday, October 05, 2000 7:37 AM
> > To: 'terij@prlnet.com'; histonet; 'Nocito, Joseph'
> > Subject: RE: Vimentin Immunostaining
> >
> > Joe,
> >
> > Is that the same peace of mind that Admiral Nelson felt?
> > As he urged his ships into battle, his aids complained of overwhelming
> > numbers of enemy ships, Nelson immediately placed the telescope to his
> > blind eye and exclaimed; Ships? I see no ships!
> >
> > Bryan
> >
> > ----------
> > From: Nocito, Joseph[SMTP:joseph_nocito@srhc.iwhs.org]
> > Sent: September 28, 2000 2:22 PM
> > To: 'terij@prlnet.com'; histonet
> > Subject: RE: Vimentin Immunostaining
> >
> > Teri,
> > we perform a vimentin on those cases that were processed at another
> > facility
> > also. We had stopped this for a while, but we were getting erroneous
> > results, so we started adding a vimentin again just to give us peace of
> > mind.
> >
> > Joe Nocito, B.S., HT(ASCP)QIHC
> > Histology Supervisor
> > Christus Santa Rosa Hospitals
> > San Antonio, Texas
> >
> > > -----Original Message-----
> > > From: terij@prlnet.com [SMTP:terij@prlnet.com]
> > > Sent: Thursday, September 28, 2000 11:31 AM
> > > To: histonet
> > > Subject: Vimentin Immunostaining
> > >
> > > We currently are doing a Vimentin immunostain on every IHC case we
> have
> > to
> > > access antigenicity. Is anyone else out there doing this? Is it
> really
> >
> > > necessary or helpful, especially considering one needs to antigen
> > retrieve
> > > this in order for it to work?
> > >
> > > Thanks,
> > >
> > > Teri Johnson
> > > Physician's Reference Laboratory
> > > Overland Park, KS
> > >
> >
>
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