B5 fixative and immunos

From:Donna Sitrin <dsitrin@unipathllc.com>

Hi all!

Somewhere during the symposium I caught wind of an innovative retrieval
method or treatment for B5 blocks to improve antigenicity. From time to
time, this same problem surfaces in my lab. Once again, I am having a
problem with CD3 staining on lymph nodes and bone marrow cores fixed in B5,
and I know there was a lot of information out there in regard to this in
particular.

I was hoping to tap your memories and that they are functioning much better
than mine.  If anyone has any information on CD3 staining on lymph nodes and
decalcified bone marrow cores fixed in B5, please jog my memory.

All help will be greatly appreciated!  (I can't talk the pathologist out of
B5 unfortunately.

Thanks in advance for all help,
Donna Sitrin
dsitrin@unipathllc.com




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