tissue processing
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From: | "Philopena, Jennifer" <jennifer.philopena@canji.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi. I work with a Shandon Citadel 2000 and I (try to) process rat and mouse
tissues (liver, lung, spleen, kidney, brain, heart). The livers and spleens
that I process are consistently very dry. This is my procedure: 24 hours in
10% neutral buffered formalin, store in 70% reagent alcohol, 20min in 80%
reagent alcohol, 2X 20min in 95% reagent alcohol, 3X 20min in 100% reagent
alcohol, 3X 20min in Propar (Xylene substitute), 2X 20min in paraffin
(Paraplast Plus). I'd like to fix this problem with the tissues being
over-dehydrated.
Thanks.
Jennifer Philopena
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