staining to demonstrate cell death

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Good morning all,  I am sending out this message for the one of our
investigators here in my lab.  Staining for this has been demonstrated in
tissue culture but can it be done with fixed tissue in paraffin sections.
Thanks,  John
Here is his question:

I would like to look for evidence of cell viability and death (either
apoptosis or necrosis) in sections of rat tendon.  I have seen references
in the literature to staining with fluorescein diacetate to identify live
cells.  I have also seen references for using propidium iodide, trypan
blue, or acridine orange to identify dead cells.  Does anyone have
experience staining tendon tissue to look for dead and live cells?  What
methods are available, and what works best?

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G161
Ann Arbor, MI 48109-0486
my office 734-936-1635
lab office 734-763-9674

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