protocol for LacZ staining

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From:"Philopena, Jennifer" <> (by way of histonet)
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The antibody comes from 5prime->3prime, catalog #5307-556410.   it's a
rabbit polyclonal.
Deparaffin etc to DI
Trypsin digest with Zymed Digest-All (or 0.25% Trypsin)
block for 30min
incubate primary Ab for an hour, at 37C, diluted at 1:100

hope that's helpful

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