in situ PCR
<< Previous Message | Next Message >>
From: | "Philopena, Jennifer" <jennifer.philopena@canji.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Our lab uses in situ PCR slides from Perkin Elmer. We heat the slides with
the tissues (paraffin, 5um)to 60C for anywhere from 1-5 hours depending on
the tissue type. We use the Perkin Elmer kit for in situ PCR, the buffer at
2x concentration, and a pro K digestion that varies in concentration and
incubation time depending on the tisse. Obviously there's a lot of
optimizing going on here. I hope this info is helpful.
<< Previous Message | Next Message >>